反应生物学/DNMT3a甲基转移酶/DMT-21-125
商品编号:
DMT-21-125
品牌:
reactionbiology
市场价:
¥10400.00
美元价:
6240.00
产品分类:
反应底物
公司分类:
Reaction_substrate
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
DESCRIPTION:DNA(cytosine-5)-methyltransferase3a(DNMT3a)isrequiredfordenovoDNAmethylationandmethylatesatbothCpGandnon-CpGsites.
ACCESSION#:NM_175629
UniprotLink
INCLUDESAMINOACIDS:2-912(C-term.)
TAG(S):N-GST
MW:127.7kDa
EXPRESSIONSYSTEM:Insectcell/Baculovirus
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin50mMTris/HClpH8.0,125mMNaCl,3mMDTT,10%glycerol(v/v).
STORAGE:-80°C,aliquotandsnap-freezeafterfirstuse.
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品牌介绍
Reaction Biology描述:
人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11
存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355
包括酰胺酸:EZH22-746,EED2-441,SUZ122-739
标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签
分子量:228.7kDa
EXPRESSIONSYSTEM :昆虫细胞/杆状病毒
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。
储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。
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