DESCRIPTION:
HumanrecombinantEEDexpressedinE.coliwithanN-terminalGST-tag.EEDfunctionsasH3K27me3readerwithinthePRC2/EED-EZH2complexandmediatesprotein-proteininteractions.Duringdevelopment,PolycombRepressiveComplex2(PRC2)istheprincipalmethyltransferaseresponsIBLeforgeneratingtrimethylatedhistoneH3lysine-27(H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.
ACCESSION#:NM_003797
INCLUDESAMINOACIDS:2-441
TAG(S):N-terminalGST-tag
MW:77.4kDa
EXPRESSIONSYSTEM:E.Coli
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin50mMTris-HCl,pH7.5,500mMNaCl,1mMTCEP,10%glycerol(v/v).
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedproteinshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossible,butverylowvolumealiquots(<5µl)orstorageofdilutedproteinisnotrecommended.
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