DESCRIPTION:
HumanrecombinantEZH1(residues2-747;GenbankAccession#NM_001991;MW=88kDa)incomplexwithhumanrecombinantsAEBP2(2-517;NM_001114176;57kDa),EED(2-441;NM_003797;51kDa),RbAp48(2-425;NM_005610;51kDa)andSUZ12(2-739;NM_015355;86kDa).TotalcomplexMWis333.4kDa.Allproteinsarefull-length(residue2throughC-terminus)andco-expressedinaninsectcell/baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS-adenosyl-L-methionine(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3(H3K27).Duringdevelopment,PolycombRepressiveComplex2(PRC2)istheprincipalmethyltransferaseresponsIBLeforgeneratingtrimethylatedhistoneH3lysine-27(H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,functionsasthecatalyticsubunitofPRC21,6,butcanbereplacedbythehomologousEZH17,whichislesscatalyticallyactive8,9,althoughPRC2-Ezh1hasstrongrepressiveactivityontranscriptionindependentofitsmethyltransferaseactivity9.EZH1actstopreservetherepressiveH3K27me3markinembryonicstemcells,complementingtheroleofEZH28.MultiplerecentresultssuggestimportantrolesforEZH1indevelopmentalandhomeostaticprocesses,includingmaintenanceoftheleft-rightaxis10,hairfolliclehomeostasisandwoundrepair11,skeletalmuscledifferentiation12andadulthematopoiesis13.
ACCESSION#:NM_001991,NM_001114176,NM_003797,NM_005610,NM_015355
UniprotLink
INCLUDESAMINOACIDS:EZH12-747,AEBP22-517,EED2-441,RbAp482-425,SUZ122-739;Allfull-length
TAG(S):N-terminalFlag-tagonEED,allothersN-terminalHis-tag
MW:333.4kDa
EXPRESSIONSYSTEM:Insectcell/Baculovirus
SUPPLIEDAS:__µMEZH1Complex,asdefinedabove,(___µg/µltotalprotein)in20mMTris-HCl,pH7.9,150mMNaCl,2mMMgCl2,2mMDTT,20%glycerol(w/v),0.01%NP-40.
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedenzymeshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossible,butverylowvolumealiquots(<5µl)orstorageofdilutedenzymeisnotrecommended.
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SubstantialDiscountsAvailableonBulkQuantities
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