DESCRIPTION:
HumanrecombinantEZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull-length(residue2throughC-terminus)andco-expressedinaninsectcell/baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS-adenosyl-L-methionine(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3(H3K27).Duringdevelopment,PolycombRepressiveComplex2(PRC2)istheprincipalmethyltransferaseresponsIBLeforgeneratingtrimethylatedhistoneH3lysine-27(H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(>3x)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and/orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti-cancertherapies11
ACCESSION#:EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355
INCLUDESAMINOACIDS:EZH22-746,EED2-441,SUZ122-739
TAG(S):EEDhasanN-terminalstrepandFlag-tag,allothersN-terminalHis-tag
MW:228.7kDa
EXPRESSIONSYSTEM:Insectcell/Baculovirus
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris-HCl,pH8,150mMNaCl,2mMMgCl2,2mMDTT,20%glycerol(w/v),0.01%NP-40asdeterminedbyOD280.
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedproteinshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossible,butverylowvolumealiquots(<5µl)orstorageofdilutedproteinisnotrecommended.
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