DESCRIPTION:Afive-membercomplex(EZH2-Y641F,AEBP2,EED,RbAp48,SUZ12)inwhichthecatalyticsubunit(EZH2-Y641F)ismutanthumanrecombinantEZH2withphenylalanine(F)substitutedfortyrosine-641(Y641).ActsasahistoneH3lysine-27methyltransferase,generatingtrimethylatedhistoneH3lysine-27(H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression.EZH2mutationsatY641,includingY641F,areassociatedwithcertaintypesoflymphomainwhichtheyoccurasheterozygoteswiththewild-typeallele..Whilewild-typeEZH2ismostefficientatmonomethylationofunmethylatedH3K27,theY641Fmutanthaspoorefficiencyforthisfirstmethylationstepbutincreasedefficiencyintransferringthesecondandthirdmethylgroups.Thusthecombinedactionofthemutantandwild-typeenzymeproducestheelevatedlevelsofH3K27me3observedintheselymphomas.
ACCESSION#:NM_001203247,NM_001114176,NM_003797,NM_005610,NM_015355
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INCLUDESAMINOACIDS:EZH2-Y641F2-746,AEBP22-517,EED2-441,RbAp482-425,SUZ122-739
TAG(S):N-terminalFlag-tagonEED,allothersN-terminalHis-tag
MW:333.8kDa
EXPRESSIONSYSTEM:Insectcell/Baculovirus
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinsin40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,20%glycerol(w/v),3mMDTT.
STORAGE:-80°C,aliquotandsnap-freezeafterfirstuse.
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