DESCRIPTION:Full-length,untaggedhumanrecombinanthistoneH1.0expressedinE.coli.H1histonesfunctionas‘linkers’thatbindtointernucleosomalDNA(~1:1withthehistoneoctamerofthenucleosomecore),convertingnucleosomesto“chromatosomes”andpromotingtheformationofmorecompact,higher-orderchromatinstructure.HistoneH1.0,theH1variantmosthomologoustotheH5histoneofavianerythrocytes,is,alongwithhistonesH1.1-H1.5,oneofsixH1variantsfoundinterminallydifferentiatedcells.Recentworkindicatesthatasidefromplayingaroleinchromatinstructureandfunction,histoneH1.0interactswithawidearrayofnucleolarproteins,withspecificinteractionsimplyingaroleinrRNAsplicingandribosomebiogenesis.Ingeneral,phosphorylationisthepredominantH1post-translationalmodification,notablyassociatedwithcellcycleprogression,butothermodifications,includinglysineacetylation/methylation1andADP-ribosylation10,arealsoreported.CrosstalkoccursbetweenH1modificationsandthoseonotherhistonesandH1variantscanaffectparticularhistonemodifications,e.g.H1.3(mouseH1d)inhibitionofSET7/9H3K4methylation(seedatasheet),consistentwitharoleforH1intranscriptionalregulation.Indeed,gene-specifictranscriptionalregulatoryeffectsofH1variantshavebeenreported,buttheseremainlesswellstudiedthanH1’sroleinchromatinstructure.
ACCESSION#:NM_005318
UniprotLink
INCLUDESAMINOACIDS:1-194
TAG(S):None
MW:20.9kDa
EXPRESSIONSYSTEM:E.coli
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin50mMTris/HClpH7.5,750mMNaCl,1mMTCEP,10%glycerol(v/v).
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedproteinshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossIBLe,butverylowvolumealiquots(<5µl)orstorageofdilutedproteinisnotrecommended.
STORAGE:-80°C,aliquotandsnap-freezeafterfirstuse.
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