反应生物学/溴甲烷检测试剂盒/BRD-Kit-01
商品编号：
BRD-Kit-01
品牌：
reactionbiology
市场价：
¥18400.00
美元价：
11040.00
产品分类：
RACE试剂盒
公司分类：
RACE_Reagent_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Test the selectivity of any compound against a wide range of bromodomains using BromoMELT TM, Reaction Biology's thermal melt stability assay kit for bromodomain targets. The BromoMELTTM kit includes 76 bromodomains in total, representing 53 members of bromodomain family.Thermal shift assays measure the thermal stability of a target protein and the increase in protein melting temperature upon the binding of a ligand to the protein. Protein melting is useful for identifying ligands, buffer conditions, cofactors and drugs affecting protein stability.
View the BROMOMELT 2.0 Instruction Manual
•Extensive Coverage of the bromodomain family
•Test selectivity of any compound within a few hours
•All reagents included
•Fast and easy to perform in your lab
View BromoMELT data >
Kit Components
- 10x Protein stock plate, 10 uL of each protein
- Protein dilution buffer, 2.5x concentrated solution (1.2 ml)
- Reaction buffer, 5x concentrated solution (1.1 ml)
- 5000x SYPRO Orange (8 uL)
- Control compound mix (5 uL)
- 100% DMSO (10 uL)
- 1x 384 well qPCR plate
- 1x Clear seal, large
- 1x Empty 96 well plate for reaction mix dispensing
- 96 well dispensing guide Optional pre-test:
- 5x Pre-test protein (30 uL)
- 1x Clear seal, small Components required but not supplied
- Centrifuge with plate compatible baskets
- Real time PCR/qPCR instrument
- Multichannel micropipettor
- 400x Stock(s) of Test Compound in DMSO
品牌介绍
Reaction Biology描述：
人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度（residue2throughC末端）也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸（SAM）tothee-aminofunctionofproteinL-lysineresidues，specificallylysine-27ofhistoneH3（ H3K27）。在开发过程中，Polycomb Repressive Complex 2（PRC2）是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27（的H3K27me3），anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2，whichincludesaSETmethyltransferasedomain，isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2，EED，SUZ12andRbAp48，whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex（> 3×）6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression，increasedmetastasisandpoorprognosis（seereview7）.DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently，EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11
存取编号： EZH2NM_001203247，EEDNM_003797，SUZ12NM_015355
包括酰胺酸：EZH22-746，EED2-441，SUZ122-739
标记（S）：EEDhasanN末端链霉菌标记标签，其他所有N末端His标签
分子量：228.7kDa
EXPRESSIONSYSTEM ：昆虫细胞/杆状病毒
SUPPLIEDAS：Solutionofpurifiedrecombinantproteinin20mMTris盐酸，pH8,150mMNaCl，2mMMgCl 2，2mMDTT，20％甘油（w / v），0.01％NP-40asdeterminedbyOD 280。
储存：-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质，例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻，但不建议使用稀释体积太小（<5µl）的稀释蛋白质。
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