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当前位置: 首页 > 产品中心 > Reaction_substrate > Reaction Biology/NSD2-E1099K Methyltransferase/HMT-21-159
商品详细Reaction Biology/NSD2-E1099K Methyltransferase/HMT-21-159
Reaction Biology/NSD2-E1099K Methyltransferase/HMT-21-159
Reaction Biology/NSD2-E1099K Methyltransferase/HMT-21-159
商品编号: HMT-21-159
品牌: reactionbiology
市场价: ¥10400.00
美元价: 6240.00
产地: 美国(厂家直采)
公司:
产品分类: 反应底物
公司分类: Reaction_substrate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

DESCRIPTION:NSD2-E1099K is a mutant human recombinant NSD2 with lysine (K) substituted for glutamate-1099 (E1099) and expressed in Sf9 insect cells. NSD2 is a SET-domain lysine methyltransferase that exerts regulatory effects primarily through methylation of H3K36, a mark associated with gene activation. NSD2 may also act as an H4K20 methyltransferase as part of a DNA damage response. Notable for its role in the genesis of t(4; 14) translocation-positive multiple myelomas, NSD2 is also overexpressed in a wide range of human cancers, including breast, lung and colon cancers. Like cells in which translocations drive NSD2 overexpression, tumor lines bearing an NSD2-E1099K mutant allele display elevated levels of H3K36 dimethylation. A truncated, SET-domain E1099K construct (residues 955-1365) has been reported to exhibit higher methylation activity toward nucleosomes in vitro. Although observed in other cancers, the E1099K mutation appears to be most prevalent in pediatric acute lymphoblastic leukemia (ALL), the most common childhood cancer. Knockdown of NSD2-E1099K in ALL lines inhibits proliferation, implicating, as in the other cancers mentioned above, NSD2 as a promising therapeutic target in ALL.

ACCESSION #: NM_001042424

Uniprot Link

INCLUDES AMINO ACIDS: 2-1365 (end)

TAG(S): N-terminal His-tag

MW: 155.5 kDa

EXPRESSION SYSTEM: Insect cell/Baculovirus

SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).

STORAGE: -80°C, aliquot and snap-freeze after first use.

View Product Data Sheet

Substantial Discounts Available on Bulk Quantities

We also offer assay services for NSD2-E1099K.

品牌介绍
Reaction Biology描述: 人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11 存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355 包括酰胺酸:EZH22-746,EED2-441,SUZ122-739 标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签 分子量:228.7kDa EXPRESSIONSYSTEM :昆虫细胞/杆状病毒 SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。 储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。