DESCRIPTION:
HumanrecombinantSET8expressedinE.coliwithanN-terminalGSTfusion-tag.CatalyzesthetransferofmethylgroupsfromS-adenosyl-L-methionine(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallythemonomethylationoflysine-20ofhistoneH4(H4K20me1)andoflysine-382ofp53(p53K382me1).SET8andH4K20me1areessentialtochromosomecondensation,entryintomitosisandmaintenanceofgenomicstABIlityandareimplicatedinthelicensingofreplicationorigins.MonomethylationofH4K20andcellularSET8levelsoscillatewiththecellcycle,peakingattheG2/Mtransition,declininglateinmitosis,withSET8proteinbecomingnearlyundetectableinSphase.Apartfromitsroleincellcycleprogression,thereisevidenceSET8canregulatetranscriptionofspecificgenesbyH4K20monomethylationinpromoterelementsorgenebodiesandbymethylationofatranscriptionfactor,p53.MultiplelinesofevidencesuggestthatSET8maybeapromisingtargetforanti-cancertherapy.TheseincludetherequirementforSET8incellcycleprogression,SET8’sactivationofWnttargetgenes,itsnegativeregulationofthep53tumorsuppressor,theassociationofincreasedSET8levelswithbreastcancermetastasisandtheassociationofdecreasedlevelswithlongersurvivalinhepatocellularcarcinomapatients.
ACCESSION#:NM_020382
INCLUDESAMINOACIDS:190-352
TAG(S):N-terminalGSTfusion-tag
MW:45.4kDa
EXPRESSIONSYSTEM:E.coli
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTrisHCl,pH7.5,300mMNaCl,10%glycerol(v/v),1mMTCEPasdeterminedbyOD280.
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedenzymeshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossIBLe,butverylowvolumealiquots(<5µl)orstorageofdilutedenzymeisnotrecommended
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SubstantialDiscountsAvailableonBulkQuantities
