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reactionbiology(优势品牌)
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当前位置: 首页 > 产品中心 > Reaction_substrate > 反应生物学/SET8甲基转移酶/HMT-11-118
商品详细反应生物学/SET8甲基转移酶/HMT-11-118
反应生物学/SET8甲基转移酶/HMT-11-118
反应生物学/SET8甲基转移酶/HMT-11-118
商品编号: HMT-11-118
品牌: reactionbiology
市场价: ¥6900.00
美元价: 4140.00
产地: 美国(厂家直采)
公司:
产品分类: 反应底物
公司分类: Reaction_substrate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

DESCRIPTION:SET8 (aka PR-Set7) is thought to be the sole methyltransferase capable of monomethylating histone H4 lysine-20 (H4K20me1) and is essential to chromosome condensation, entry into mitosis and maintenance of genomic stability. Apart from its role in cell cycle progression, there is evidence SET8 can regulate transcription of specific genes by H4K20 monomethylation in promoter elements or gene bodies and by methylation of a transcription factor, p53 (p53K382me1). Multiple lines of evidence suggest that SET8 may be a promising target for anti-cancer therapy. These include the requirement for SET8 in cell cycle progression, SET8’s activation of Wnt target genes, its negative regulation of the p53 tumor suppressor, the association of increased SET8 levels with breast cancer metastasis and the association of decreased levels with longer survival in hepatocellular carcinoma patients.

ACCESSION #: NM_020382

Uniprot Link

INCLUDES AMINO ACIDS: 190-352 (C-term.)

TAG(S): N-terminal His-tag

MW: 22.0 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10% glycerol (v/v), 1 mM TCEP

STORAGE: -80°C, aliquot and snap-freeze after first use.

View Product Data Sheet

Substantial Discounts Available on Bulk QuantitiesWe also offer assay services for SET8.
品牌介绍
Reaction Biology描述: 人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11 存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355 包括酰胺酸:EZH22-746,EED2-441,SUZ122-739 标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签 分子量:228.7kDa EXPRESSIONSYSTEM :昆虫细胞/杆状病毒 SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。 储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。