DESCRIPTION:
Human recombinant SMYD3 expressedwith an N-terminal His-tag, in E. coli. Catalyzes the transfer of methyl groupsfrom S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysineresidues, particularly in histones H4 and H3, although other activities havebeen reported. Lysine methyltransferases of the SMYD family are unusual in thattheir SET domains are split in two by insertion of a myeloid-Nervy-DEAF-1(MYND) domain in the primary amino acid sequence1-3. SMYD3 (SET andMYND domain containing protein 3) has been described as a histone H3 lysine-4(H3K4) and histone H4 lysine-20 (H4K20) methyltransferase (HMT), although morerecent evidence suggests that it may be a histone H4 lysine-5 (H4K5) HMT. Smyd3has also been shown to methylate K260 of MAP3K24,5. SMYD3 isoverexpressed in a number of cancers, including breast and liver.
ACCESSION #: NM_001167740
INCLUDES AMINO ACIDS: 2-428 (C-terminus)
TAG(S): N-terminal HIS-tag
MW:50.1kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris HCl,pH 8.0, 250 mM NaCl, 1 mM TCEP, 10% glycerol as determined by OD280
STORAGE: -70°C. Thaw quickly and store on ice before use. Theremaining, unused, undiluted enzyme should be refrozen quickly by, for example,snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing andstorage of diluted enzyme is not recommended.
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Substantial Discounts Available onBulk Quantities
