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reactionbiology(优势品牌)
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当前位置: 首页 > 产品中心 > Reaction_substrate > 反应生物学/BAZ2B(GST)/RD-11-189
商品详细反应生物学/BAZ2B(GST)/RD-11-189
反应生物学/BAZ2B(GST)/RD-11-189
反应生物学/BAZ2B(GST)/RD-11-189
商品编号: RD-11-189
品牌: reactionbiology
市场价: ¥6400.00
美元价: 3840.00
产地: 美国(厂家直采)
公司:
产品分类: 反应底物
公司分类: Reaction_substrate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

DESCRIPTION:Human recombinant BAZ2B bromodomain. In addition to a bromodomain, native BAZ2B comprises several other domains (DDT, PHD, MBD (methyl-CpG-binding domain)) that may interact with the DNA and histones of chromatin and its associated proteins, including chromatin remodeling complexes. The BAZ2B bromodomain binds various Lys(Ac) residues in singly acetylated histone peptide microarrays (histones H1.4, H2A, H2B, H3, H4) with binding to histone H3 K14(Ac) confirmed in solution by isothermal titration calorimetry (ITC). An allele of the BAZ2B locus shows an association with increased risk for sudden cardiac death.

ACCESSION #: NM_013450

Uniprot Link

INCLUDES AMINO ACIDS: 2054-2168

TAG(S): N-terminal GST-tag

MW: 40.4 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 50 mM HEPES, pH 7.0, 150 mM NaCl, 1 mM TCEP, 10 % glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted enzyme is not recommended.

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Substantial Discounts Available on Bulk Quantities

品牌介绍
Reaction Biology描述: 人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11 存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355 包括酰胺酸:EZH22-746,EED2-441,SUZ122-739 标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签 分子量:228.7kDa EXPRESSIONSYSTEM :昆虫细胞/杆状病毒 SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。 储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。