4000-520-616
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reactionbiology(优势品牌)
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商品详细反应生物学/BRD3-1(GST)/RD-11-163
反应生物学/BRD3-1(GST)/RD-11-163
反应生物学/BRD3-1(GST)/RD-11-163
商品编号: RD-11-163
品牌: reactionbiology
市场价: ¥6400.00
美元价: 3840.00
产地: 美国(厂家直采)
公司:
产品分类: 反应底物
公司分类: Reaction_substrate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

DESCRIPTION:Human recombinant BRD3, bromodomain-1 expressed as an N-terminal GST fusion protein. BRD3, like other human members of the BET family of chromatin-binding proteins (BRD2, BRD4, BRDT), comprises two bromodomains, protein modules that bind epsilon-N-acetyllysine residues. Recent results suggest an important role for BRD3 in linking acetylation of both histones and non-histone proteins to gene transcription. When overexpressed in 293 cells, BRD3, along with BRD2, binds the hyperacetylated chromatin of transcribed genes, regions enriched in acetylated histone H4 lysine-5 (H4K5Ac), H4K12Ac, H3K14Ac, but deficient in H4K16Ac and H3K9me. In an in vitro RNA polymerase II transcription system, binding of either BRD3 or BRD2 to a chromatin template assembled with hyperacetylated histones enabled transcription through the nucleosomes. In addition to acetylated histones, BRD3-1 (BRD3 bromodomain 1) has been found to bind the hematopoietic transcription factor GATA1 and to enhance its chromatin binding and activation of target genes. The BET family inhibitor, I-BET151, has shown efficacy in mouse models of MLL-fusion leukemias, displacing BRD3 and BRD4 from chromatin and inhibiting transcription of genes, e.g. BCL2, upregulated by the MLL-fusions.

ACCESSION #: NM_007371

Uniprot Link

INCLUDES AMINO ACIDS: 24-144

TAG(S): GST

MW: 41.4 kDa

EXPRESSION SYSTEM: E. coli

SUPPLIED AS: Solution of purified recombinant protein in 20 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1.0 mM TCEP, 10% glycerol (w/v) as determined by OD280

STORAGE:

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Substantial Discounts Available on Bulk Quantities

We also offer assay services for BRD3-1 (GST).

品牌介绍
Reaction Biology描述: 人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11 存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355 包括酰胺酸:EZH22-746,EED2-441,SUZ122-739 标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签 分子量:228.7kDa EXPRESSIONSYSTEM :昆虫细胞/杆状病毒 SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。 储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。