DESCRIPTION:Human recombinant BRPF1a bromodomain (longer BRPF1 isoform, isoform 1). Relative to BRPF1b (BRPF1 Isoform 2), the BRRPF1a bromodomain contains a six amino acid insert, EVTELD, after residue 660. A scaffolding component of the MOZ/MORF histone acetyltransferase (HAT) complex and also an HBO1-BRPF1 HAT complex, native BRPF1b contains, in addition to its acetyllysine-binding bromodomain, two other presumed histone/chromatin binding domains, a PWWP domain and a PHD zinc-finger. The BRPF1 bromodomain binds multiple acetyllysines in histone N-terminal tails, with preference for H2AK5Ac, H4K12Ac and H3K14Ac. BRPF1 forms a complex with MOZ-TIF2 fusion proteins formed due to chromosomal translocations associated with acute myeloid leukemia (AML). Depletion of BRPF1 interferes with the MOZ-TIF2-driven transformation and upregulation of HOX gene expression, suggesting that BRPF1 could be a therapeutic target for AML.
ACCESSION #: NM_001003694
Uniprot Link
INCLUDES AMINO ACIDS: 627-746
TAG(S): N-terminal His-tag
MW: 16.9 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
We also offer assay services for BRPF1a (His).
