DESCRIPTION:
Human recombinant PB1-1 bromodomain expressed as an N-terminal GST-fusion protein in E.coli. PB1, a chromatin targeting component of the PBAF chromatin-remodeling complex, comprises six tandem bromodomains. Mutations in PB1, including PB1-1 occur frequently in renal cancers. Among the acetylated lysines of histone N-terminal tails, PB1-1 binds preferentially to H3K14aC. Crystal structures of PB1-1, both in complex with H3K14Ac peptide and isolation have been determined.
ACCESSION #:NM_018313
Uniprot Link
INCLUDES AMINO ACIDS:23-156
TAG(S):N-terminal GST tag
MW: 43.0 kDa
EXPRESSION SYSTEM: E.coli
SUPPLIED AS:Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10 % glycerol
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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