反应生物学/TM-2-51/EI-HDC-014
商品编号：
EI-HDC-014
品牌：
reactionbiology
市场价：
¥1300.00
美元价：
780.00
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TM-2-51
Highly potentand isozyme selective activator of recombinant HDAC8. Increases the activity of HDAC8 by a factor of12 at 10 µM.No activation of other HDACs is observed. Rescues the activity of catalyticallycompromised HDAC8 mutants in vitro.Amount:10mgPhysical Properties:Beigepowder
Highly potentand isozyme selective activator of recombinant HDAC8. Increases the activity of HDAC8 by a factor of12 at 10 µM.No activation of other HDACs is observed. Rescues the activity of catalyticallycompromised HDAC8 mutants in vitro.Amount:10mgPhysical Properties:BeigepowderMW=256.3 C14H12N2OS
Analysis:>98%(TLC); NMR (Conforms)
Solubilization:May be dissolved in DMSO (50 mg/ml); or ethanol (8 mg/ml)
Storage:Storedesiccated as supplied at -20°C for up to 2 years. Store solutions at-20°C for up to 3 months.
品牌介绍
Reaction Biology描述：
人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度（residue2throughC末端）也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸（SAM）tothee-aminofunctionofproteinL-lysineresidues，specificallylysine-27ofhistoneH3（ H3K27）。在开发过程中，Polycomb Repressive Complex 2（PRC2）是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27（的H3K27me3），anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2，whichincludesaSETmethyltransferasedomain，isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2，EED，SUZ12andRbAp48，whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex（> 3×）6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression，increasedmetastasisandpoorprognosis（seereview7）.DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently，EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11
存取编号： EZH2NM_001203247，EEDNM_003797，SUZ12NM_015355
包括酰胺酸：EZH22-746，EED2-441，SUZ122-739
标记（S）：EEDhasanN末端链霉菌标记标签，其他所有N末端His标签
分子量：228.7kDa
EXPRESSIONSYSTEM ：昆虫细胞/杆状病毒
SUPPLIEDAS：Solutionofpurifiedrecombinantproteinin20mMTris盐酸，pH8,150mMNaCl，2mMMgCl 2，2mMDTT，20％甘油（w / v），0.01％NP-40asdeterminedbyOD 280。
储存：-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质，例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻，但不建议使用稀释体积太小（<5µl）的稀释蛋白质。
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