反应生物学/DMOG/EI-DEM-004
商品编号：
EI-DEM-004
品牌：
reactionbiology
市场价：
¥1140.00
美元价：
684.00
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DMOG
N-(2-Methoxy-2-oxoacetyl)glycinemethyl ester; DimethyloxalylglycineDMOG inhibits PHD or prolyl hydroxylase domain-containing proteins, activatingHIF1a and inhibiting JMJD2A (IC50 = 2.5 mM). Displaysneuroprotective effects. Inhibits neuronal cell death caused by neurotrophindeprivation. Prevents activation of the ATR/CHK1/p53 pathway andsuppresses apoptosis induced by DNA damage.Physical Properties: White tooff-white solid; MP=46-48°C
N-(2-Methoxy-2-oxoacetyl)glycinemethyl ester; DimethyloxalylglycineDMOG inhibits PHD or prolyl hydroxylase domain-containing proteins, activatingHIF1a and inhibiting JMJD2A (IC50 = 2.5 mM). Displaysneuroprotective effects. Inhibits neuronal cell death caused by neurotrophindeprivation. Prevents activation of the ATR/CHK1/p53 pathway andsuppresses apoptosis induced by DNA damage.Physical Properties: White tooff-white solid; MP=46-48°CMW=175.1 C6H9NO5
Analysis: >98% (TLC); NMR(Conforms)
Solubilization: May be dissolved in DMSO (30 mg/ml), ethanol (25 mg/ml),or water (30 mg/ml)
Storage: Store desiccated assupplied at -20°C for up to 2 years. Store solutions at -20°C for up to 1 month.
Amount:10 mg品牌介绍
Reaction Biology描述：
人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度（residue2throughC末端）也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸（SAM）tothee-aminofunctionofproteinL-lysineresidues，specificallylysine-27ofhistoneH3（ H3K27）。在开发过程中，Polycomb Repressive Complex 2（PRC2）是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27（的H3K27me3），anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2，whichincludesaSETmethyltransferasedomain，isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2，EED，SUZ12andRbAp48，whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex（> 3×）6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression，increasedmetastasisandpoorprognosis（seereview7）.DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently，EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11
存取编号： EZH2NM_001203247，EEDNM_003797，SUZ12NM_015355
包括酰胺酸：EZH22-746，EED2-441，SUZ122-739
标记（S）：EEDhasanN末端链霉菌标记标签，其他所有N末端His标签
分子量：228.7kDa
EXPRESSIONSYSTEM ：昆虫细胞/杆状病毒
SUPPLIEDAS：Solutionofpurifiedrecombinantproteinin20mMTris盐酸，pH8,150mMNaCl，2mMMgCl 2，2mMDTT，20％甘油（w / v），0.01％NP-40asdeterminedbyOD 280。
储存：-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质，例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻，但不建议使用稀释体积太小（<5µl）的稀释蛋白质。
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