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reactionbiology(优势品牌)
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当前位置: 首页 > 产品中心 > Reaction_substrate > 反应生物学/SirtAct(CAY10591)/EI-SRT-005
商品详细反应生物学/SirtAct(CAY10591)/EI-SRT-005
反应生物学/SirtAct(CAY10591)/EI-SRT-005
反应生物学/SirtAct(CAY10591)/EI-SRT-005
商品编号: EI-SRT-005
品牌: reactionbiology
市场价: ¥1960.00
美元价: 1176.00
产地: 美国(厂家直采)
公司:
产品分类: 反应底物
公司分类: Reaction_substrate
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

SirtAct

2-?Amino-?N-?cyclopentyl-?1-?(3-?methoxypropyl)-?1H-?pyrrolo[2,?3-?b]quinoxaline-?3-?carboxamide;CAY10591

SirtAct is a Sirt1 activator (233% at 10 mM using the BIOMOL Fluor-de-Lys assay)1 Suppresses TNFa release from THP-1 cells and stimulates adipogenesis in 3T3L1 cells.SIRT1 stimulation by SirtAct leads to decreased tissue factor mediatedthrombogenicity in mice. Inhibits NFkB activation and reduces inflammatory cytokine production in laminapropria mononuclear cells from IBD patients3. Prevents and curesexperimental colitis in a mouse model.

Physical Properties: Olive green powder

MW=367.4 C20H25N5O2

Analysis: 98% (TLC); NMR (Conforms)

Solubilization: May be dissolved in DMSO (50 mg/ml); or ethanol (20 mg/ml,warm)

Storage: Store desiccated assupplied at -20°C for up to 2 years. Store solutions at-20°C for up to 3 months.

Amount: 5mg

品牌介绍
Reaction Biology描述: 人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11 存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355 包括酰胺酸:EZH22-746,EED2-441,SUZ122-739 标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签 分子量:228.7kDa EXPRESSIONSYSTEM :昆虫细胞/杆状病毒 SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。 储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。