SET1A Complex

来自 : 发布时间：2024-12-25

SET1A Complex is a complex catalyzing the transcription-activating methylation of histone H3 lysine-4 and comprising a catalytic, SET domain-containing subunit, SET1A (SETD1A), plus the subunits of an activity-enhancing sub-complex—WDR5, RbBP5, Ash2L and DPY-30 (2 copies). In contrast to the H3K4-methylating activities of other MLL/SET1 family members that are more narrowly focused (e.g. MLL1 and MLL2 and the HOX genes), human SET1-containing complexes (SET1A and SET1B) appear to be responsible for widespread H3K4 trimethylation. Nevertheless, there is evidence that specific targeting of SET1A for inhibition might be a fruitful therapeutic approach to some diseases, including osteoarthritis and colon cancer. ACCESSION #: NM_014712, NM_017588, NM_005057, NM_001105214, NM_0325742 Uniprot Link

INCLUDES AMINO ACIDS: SET1A 1418-1707 (C-term.), WDR5 22-334 (C-term.), RbBP5 1-538 (C-term.), Ash2L 2-534 (C-term.), DPY-30 1-99 (C-term.) TAG(S): N-terminal His-tag on all subunits, but SET1A, which is GST-tagged MW: 249.0 kDa EXPRESSION SYSTEM: E. coli SUPPLIED AS:Solution of purified recombinant proteins in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM TCEP (tris(2-carboxyethyl)phosphine HCl), 10% (w/v) glycerol, 1 µM ZnCl2. STORAGE:-80°C, aliquot and snap-freeze after first use. SET1A Complex Product Data Sheet

Cat. #:HMT-15-116Size:50 µg